RecA-Mediated Affinity Capture: A Method for Full-Length cDNA Cloning
نویسندگان
چکیده
منابع مشابه
RecA-mediated affinity capture: a method for full-length cDNA cloning.
We describe an improved method for rapid cloning of full-length cDNA from cDNA libraries. This approach is based on the ability of Escherichia coli RecA protein to form a stable nucleoprotein complex with a linear single-stranded DNA probe and homologous sequences in circular double-stranded DNA. Hybridization of RecA-coated biotinylated DNA probes to homologous plasmid DNA creates triple-stran...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99274rr06